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WiCell Research Institute Inc
h9 human embryonic stem cells hescs ![]() H9 Human Embryonic Stem Cells Hescs, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/h9 human embryonic stem cells hescs/product/WiCell Research Institute Inc Average 99 stars, based on 1 article reviews
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WiCell Research Institute Inc
h9 human embryonic stem cells ![]() H9 Human Embryonic Stem Cells, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/h9 human embryonic stem cells/product/WiCell Research Institute Inc Average 99 stars, based on 1 article reviews
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WiCell Research Institute Inc
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Journal: Biofabrication
Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing
doi: 10.1088/1758-5090/ae2718
Figure Lengend Snippet: Culturing human embryonic stem cells in ColTubes. (A) Phase-contrast images of H9 hESCs cultured in ColTubes at days 0, 1, 3, 5, and 7. (B) Live/Dead staining of H9 cells inside the tubes and released from the tubes on day 7. (C) Flow cytometry analysis shows that 99.6% of cells harvested on day 7 are Calcein AM-positive (live cells). (D) Flow cytometry analysis confirms that majority cells on day 7 express pluripotency markers OCT4 and Nanog.
Article Snippet: For a typical cell culture,
Techniques: Cell Culture, Staining, Flow Cytometry
Journal: Biofabrication
Article Title: Collagen hydrogel tube microbioreactors for cell and tissue manufacturing
doi: 10.1088/1758-5090/ae2718
Figure Lengend Snippet: Differentiating H9 hESCs into Cardiomyocytes in ColTubes. (A) The cardiomyocyte production protocol. H9 hESCs are processed into ColTubes and expanded in E8 medium, followed by mesoderm induction for 1 d, cardiomyocyte differentiation from days 2–11, and metabolic enrichment from days 11–18. (B) Phase-contrast and fluorescent images of cells in ColTubes on days 0, 1, 3, 5, 7, 11, 15, and 18. Cardiomyocytes are cTnT-positive.
Article Snippet: For a typical cell culture,
Techniques: